KANDUNGAN FENOL, TANIN, DAN TOTAL KOLONI BAKTERI PENDEGRADASI FENOL PADA LAHAN GAMBUT YANG DIRESTORASI HIDROLOGI

Octavia Yesie Andeni, Evi Gusmayanti, Gusti Zakaria Anshari

Abstract


Phenolic compounds are one of the organic materials that can inhibit the decomposition process of peat, are toxic to plants, and difficult to degrade. Degradation of phenolic content can be done enzymatically by phenol-degrading bacteria. The purpose of this study was to compare the content of phenol, tannin, and total colonies of phenol-degrading bacteria on mixed agricultural land at upstream and downstream of the canal blocking built through the hydrological restoration program. The research was conducted from September 2022 to January 2023. Soil samples were taken from two transects (upstream and downstream of canal blocking) using a peat drill.  The samples were analyzed to measure phenol, tannin, and bacterial content.  The results showed insignificant differences in the content of phenol and tannin in peat samples collected from the two transects. The pH, water content, and C-organic content were found in a similar pattern. Total colonies of phenol-degrading bacteria tended to be higher in the transect located upstream compared to the downstream of canal blocking.

Keywords: phenol, phenol-degrading bacteria, tannin.

INTISARI

Senyawa fenolik merupakan salah satu bahan organik yang dapat menghambat proses dekomposisi gambut, bersifat racun bagi tanaman, dan sulit terdegradasi. Degradasi kandungan fenolik dapat dilakukan secara enzimatik oleh bakteri pendegradasi fenol. Tujuan penelitian ini adalah membandingkan kandungan fenol, tanin, dan total koloni bakteri pendegradasi fenol pada lahan pertanian campuran hulu dan hilir sekat kanal yang dibangun melalui program restorasi hidrologi. Penelitian dilaksanakan pada bulan September 2022 hingga Januari 2023. Sampel tanah diambil pada dua transek (hulu dan hilir sekat kanal) dengan menggunakan alat bor gambut. Sampel dianalisis untuk mengukur kandungan fenol, tanin, dan bakteri. Hasil penelitian menunjukkan adanya perbedaan yang tidak signifikan kandungan fenol dan tanin pada sampel gambut yang dikumpulkan dari kedua transek. PH, kadar air, dan kandungan C-organik ditemukan dalam pola yang sama. Total koloni bakteri pendegradasi fenol cenderung lebih tinggi pada transek yang terletak di bagian hulu dibandingkan di bagian hilir sekat saluran.

 Kata kunci: fenol, bakteri pendegradasi fenol, tanin


Full Text:

PDF

References


Chen, J., Luo, Y., Van Groenigen, K. J., Hungate, B. A., Cao, J., Zhou, X., & Wang, R. Wu. 2018. A keystone microbial enzyme for nitrogen control of soil carbon storage. Science Advances, 4(8), 2–8. https://doi.org/10.1126/sciadv.aaq1689

Jian, S., Li, J., Chen, J., Wang, G., Mayes, M. A., Dzantor, K. E., Hui, D., & Luo, Y. 2016. Soil extracellular enzyme activities, soil carbon and nitrogen storage under nitrogen fertilization: A meta-analysis. Soil Biology and Biochemistry, 101, 32–43. https://doi.org/10.1016/j.soilbio.2016.07.003

Kanerva S, Kitunen V, Loponen J, Smolander A. 2008. Phenolic compounds and terpenes in soil organic horizon layers under silver birch, Norway spruce and scots pine. Biol Fertil Soils. 44:547–556.

Li, T., Bu, Z., Liu, W., Zhang, M., Peng, C., Zhu, Q., Shi, S., & Wang, M. 2019. Weakening of the ‘enzymatic latch’ mechanism following long-term fertilization in a minerotrophic peatland. Soil Biology and Biochemistry, 136(July), 107528. https://doi.org/10.1016/j.soilbio.2019.107528

Lim, T.Y., Lim,Y.Y., and Yule,C.M, 2014. Bioactivity of leaves of Macaranga species in tropical peat swamp and non-peat swam penvironments. J.Trop. Forest Sci. 26,134–141.

Masni, T. E., Linda, T. M., dan Fibriarti, B. L. 2020. Studi Pertumbuhan dan Degradasi Fenol oleh Kultur Tunggal Aksinomisetes dari Tanah Gambut. Al-Kauniyah: Jurnal Biologi, 13(1), 95-104.

Meng, C., Tian, D., Zeng, H., Li, Z., Chen, H. Y. H., & Niu, S. 2020. Global meta-analysis on the responses of soil extracellular enzyme activities to warming. Science of the Total Environment 705: 135992. https://doi.org/10.1016/j.scitotenv.2019.135992

Min K, Freeman C, Kang H, S.U. Choi. 2015. The regulation by phenolic compounds of soil organic matter dynamics under a changing environment. Bio Med Res 2015:1-11

Polak, B. 1975.Character and occurrence of peat deposits in the Malaysian tropics.In G.J. Barstra, and W.A. Casparie (Eds.).Modern Quaternary Research in Southeast Asia. Balkema, Rotterdam.

Saraswati, R., Husen, E., Simanungkalit R.D.M. 2007. Pengambilan Contoh Tanah untuk Analisis Mikroba. In: Metode Analis Biologi Tanah. Balitbang, Sumberdaya Lahan Pertanian, Bogor.

Stevenson, F. J. 1994. Humus Chemistry, Genesis, Composition, Reaction. New York: A Wiley Internscience and Sons. 496 pp.

Supriyanti, A. & Sumardi. 2023. Review : Mikroba Pendegradasi Lignoselulosa Dari Tanah Gambut. Jurnal Pertanian Agros 25(3): 2928-2941

Zak, D., Roth, C., Unger, V., Goldhammer, T., Fenner, N., Freeman, C., & Jurasinski, G. (2019). Unraveling the Importance of Polyphenols for Microbial Carbon Mineralization in Rewetted Riparian Peatlands. Frontiers in Environmental Science, 7(October), 1–14. https://doi.org/10.3389/fenvs.2019.00147




DOI: http://dx.doi.org/10.37159/jpa.v26i1.4050

Refbacks

  • There are currently no refbacks.


 

Lisensi Creative Commons
Karya ini dilisensikan di bawah Creative Commons Attribution-ShareAlike 4.0 International License .

 E-ISSN 2528-1488; P-ISSN 1411-0172